SrasV1, Main, Exploration, bibRecord, 001663

Development of a single nucleotide polymorphism DNA microarray for the detection and genotyping of the SARS coronavirus.

Identifieur interne : 001663 ( Main/Exploration ); précédent : 001662; suivant : 001664

Development of a single nucleotide polymorphism DNA microarray for the detection and genotyping of the SARS coronavirus.

Auteurs : Xi Guo [République populaire de Chine] ; Peng Geng ; Quan Wang ; Boyang Cao ; Bin Liu

Source :

Journal of microbiology and biotechnology [ 1738-8872 ] ; 2014.

RBID : pubmed:24950883

Descripteurs français

KwdFr :
- Animaux, Humains, Polymorphisme de nucléotide simple, Séquençage par oligonucléotides en batterie (), Techniques de génotypage (), Virologie (), Virus du SRAS (), Virus du SRAS (génétique), Épidémiologie moléculaire ().
MESH :
- génétique : Virus du SRAS.
- Animaux, Humains, Polymorphisme de nucléotide simple, Séquençage par oligonucléotides en batterie, Techniques de génotypage, Virologie, Virus du SRAS, Épidémiologie moléculaire.

English descriptors

KwdEn :
- Animals, Genotyping Techniques (methods), Humans, Molecular Epidemiology (methods), Oligonucleotide Array Sequence Analysis (methods), Polymorphism, Single Nucleotide, SARS Virus (classification), SARS Virus (genetics), Virology (methods).
MESH :
- classification : SARS Virus.
- genetics : SARS Virus.
- methods : Genotyping Techniques, Molecular Epidemiology, Oligonucleotide Array Sequence Analysis, Virology.
- Animals, Humans, Polymorphism, Single Nucleotide.

Abstract

Severe acute respiratory syndrome (SARS), a disease that spread widely in the world during late 2002 to 2004, severely threatened public health. Although there have been no reported infections since 2004, the extremely pathogenic SARS coronavirus (SARS-CoV), as the causative agent of SARS, has recently been identified in animals, showing the potential for the re-emergence of this disease. Previous studies showed that 27 single nucleotide polymorphism (SNP) mutations among the spike (S) gene of this virus are correlated closely with the SARS pathogenicity and epidemicity. We have developed a SNP DNA microarray in order to detect and genotype these SNPs, and to obtain related information on the pathogenicity and epidemicity of a given strain. The microarray was hybridized with PCR products amplified from cDNAs obtained from different SARS-CoV strains. We were able to detect 24 SNPs and determine the type of a given strain. The hybridization profile showed that 19 samples were detected and genotyped correctly by using our microarray, with 100% accuracy. Our microarray provides a novel method for the detection and epidemiological surveillance of SARS-CoV.

DOI: 10.4014/jmb.1404.04024
PubMed: 24950883

Affiliations:

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Serveur d'exploration SRAS

Development of a single nucleotide polymorphism DNA microarray for the detection and genotyping of the SARS coronavirus.

Development of a single nucleotide polymorphism DNA microarray for the detection and genotyping of the SARS coronavirus.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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